Core Product Components
NK Induction and Expansion Kit: Pure-factor induction, feeder-free, no cell counting required.
Immune-Repair Medium 2.0: Dual repair for cells and plasma, broad blood sample compatibility.
Serum-Free Medium 5.0: 5×10⁶/mL seeding density, 5-day viability maintenance after medium supplementation.
FEP Culture Bag: FEP material · high oxygen permeability · low water vapor transmission · high light transmittance · excellent biocompatibility
Product Specifications
| Culture scale | Core catalog number | Applicable sample volume | Core components | Harvested cell number |
| 1 L Ultra-small-scale System | AN0104.3/NC0113-1 | 5 mL | 5 mL Kit + 50 mL Immune-Repair Medium 2.0 | 2.5-4.0 billion |
| 1 L Standard System | AN0104.4/NC0113-2 | 15 mL | 15 mL Kit + 100 mL Immune-Repair Medium 2.0 | 3.0-5.0 billion |
| 2 L System | AN0104/0104.2/NC0113-3 | 30/50 mL | 30/50 mL Kit + 200 mL Immune-Repair Medium 2.0 | 6.0-10.0 billion |
| 6 L System | AN0104.5/NC0113-3 | 60 mL | 60 mL Kit + 200 mL Immune-Repair Medium 2.0 | 20.0-30.0 billion |
| 12-30 L Extra-large-scale System | AN0104.6/NC0113-3×2 | 70/80 mL | 70/80 mL Kit + 400 mL Immune-Repair Medium 2.0 | 50.0-100.0 billion |
Note: All culture systems come with basal medium — Serum-Free Immune Cell Medium 5.0.
Technical Core: Dual Repair Mechanism
Immune-Repair Medium 2.0 can repair PBMCs, rendering them susceptible to activation, while enabling plasma repair: neutralize the damage to the culture system caused by anticoagulants in plasma and replenish deficient plasma components, ensuring stable culture outcomes for samples with poor-quality plasma.
Data Comparison (1.0 vs 2.0)
| Sample Type | Plasma / Plasma Substitute | Expansion Fold (Improvement) | NK Phenotype (Improvement) |
| Peripheral Blood | Peripheral Blood Plasma | 208→228 +9.6% | 98.8%→99.2% +0.39% |
| D-brand plasma substitute | 225→350 +55.6% | 90.3%→96.6% +6.34% | |
| Cord Blood｜NK Phenotype: 28.0% | Cord Blood | 246→594 +141.5% | 96.3%→97.9% +1.6% |
| H-brand plasma substitute | 389→710 +82.5% | 96.7%→97.6% +0.9% |
Case Showcase Full-scale ：
Culture Measured Data
Ultra-small-scale: 1 L (Peripheral Blood / Cord Blood) Expansion: 357-572-fold Harvest: 2.5-4.6 billion cells Purity: 96.63%-99.19%；
1 L Standard System (Peripheral Blood / Cord Blood) Expansion: 125-225-fold Harvest: 2.5-4.5 billion cells Purity: 99.56%-99.82%；
2 L System (Peripheral Blood / Cord Blood) Expansion: 150-153-fold Harvest: 9.0-9.2 billion cells Purity: 98.57%-99.11%；
6 L System (Cord Blood) Expansion: 210-fold Harvest: 25.2 billion cells Purity: 93.88%.
Maintenance of High Cytotoxic Capacity of NK Cells
